Full Article

Targeted optical injection of gold nanoparticles into single mammalian cells

  1. Craig McDougall2,†,*,
  2. David J. Stevenson1,2,†,
  3. Christian T. A. Brown2,
  4. Frank Gunn-Moore1,
  5. Kishan Dholakia2

Article first published online: 14 JUL 2009

DOI: 10.1002/jbio.200910030

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 2, Issue 12, pages 736–743, December 2009

Additional Information

How to Cite

McDougall, C., Stevenson, D. J., Brown, C. T. A., Gunn-Moore, F. and Dholakia, K. (2009), Targeted optical injection of gold nanoparticles into single mammalian cells. Journal of Biophotonics, 2: 736–743. doi: 10.1002/jbio.200910030

Author Information

  1. 1

    School of Biology, Bute Medical Building, University of St Andrews, St Andrews, KY16 9TS, UK

  2. 2

    SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK

  1. †

    Authors contributed equally to this work

*Phone: +44(0)1334 463439

Publication History

  1. Issue published online: 2 DEC 2009
  2. Article first published online: 14 JUL 2009
  3. Manuscript Accepted: 12 JUN 2009
  4. Manuscript Revised: 8 JUN 2009
  5. Manuscript Received: 1 APR 2009

Funded by

  • UK Engineering and Physical Sciences Research Council
  • EU Network of Excellence Photonics 4 Life
  • a Wellcome Trust equipment grant

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (549K)

Keywords:

  • photoporation;
  • optical tweezing;
  • optical trapping;
  • optical injection

Abstract

We present an all optical technique for the targeted delivery of single 100 nm diameter gold nanoparticles into a specified region of the interior of an individual mammalian cell through a combination of optical tweezing and optical injection. The internalisation of the nanoparticle is verified by confocal laser scanning microscopy and confocal laser scanning reflectance microscopy. This represents the first time that nano sized particles have been tweezed and optically injected into mammalian cells using only light, and provides a novel methodology for internalising nanosphere based biosensors within specific intracellular regions of a mammalian cell. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Get PDF (549K)