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Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light–cell interaction

  1. Stoyan Tanev,
  2. Wenbo Sun2,
  3. James Pond3,
  4. Valery V. Tuchin4,
  5. Vladimir P. Zharov5

Article first published online: 10 AUG 2009

DOI: 10.1002/jbio.200910039

Issue

Journal of Biophotonics

Journal of Biophotonics

Special Issue: Towards in vivo Flow Cytometry

Volume 2, Issue 8-9, pages 505–520, September 2009

Additional Information

How to Cite

Tanev, S., Sun, W., Pond, J., Tuchin, V. V. and Zharov, V. P. (2009), Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light–cell interaction. J. Biophoton., 2: 505–520. doi: 10.1002/jbio.200910039

Author Information

  1. 2

    Science Systems and Applications Inc., 10210 Greenbelt Road, Suite 600, Lanham, MD 20706, USA

  2. 3

    Lumerical Solutions, Suite 201–1290 Homer Street, Vancouver, BC V6B 2Y5, Canada

  3. 4

    Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia, Institute of Precise Mechanics and Control of RAS, Saratov 410028, Russia

  4. 5

    Phillips Classic Laser and Nanomedicine Laboratories, Arkansas University of Medical Science, 4301 W. Markham St., Little Rock, AR 72205 USA

Publication History

  1. Issue published online: 9 SEP 2009
  2. Article first published online: 10 AUG 2009
  3. Manuscript Accepted: 18 JUN 2009
  4. Manuscript Received: 16 MAY 2009

Funded by

  • RF President's “Supporting of Leading Scientific Schools”. Grant Number: 224014 PHOTONICS4LIFE-FP7-ICT-2007-2 (2008-2013)
  • PF Program on the Development of High School Potential. Grant Numbers: NSHA-208.2008.2 (2008–2009), 2.1.1/4989, 2.2.1.1/2950
  • National Institute of Health. Grant Numbers: EB000873, EB009239, CA131164, CA139373

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Keywords:

  • finite-difference time-domain (FDTD) method;
  • light scattering;
  • cytometry;
  • gold nanoparticle;
  • optical phase contrast microscopy;
  • refractive index matching;
  • image contrast enhancement;
  • nanoscale cell imaging

Abstract

The formulation of the finite-difference time-domain (FDTD) approach is presented in the framework of its potential applications to in-vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive-index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for in-vivo flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open up a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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