In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles

Authors

  • Ekaterina I. Galanzha,

    1. Institute of Optics and Biophotonics, Saratov State University, Saratov 410012, Russia
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  • Mimi S. Kokoska,

    1. Richard L. Roudebush VA Medical Center and Indiana University School of Medicine, Department of Otolaryngology-Head & Neck Surgery, Indianapolis, IN 46202, USA
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  • Evgeny V. Shashkov,

    1. Winthrop P. Rockefeller Cancer Institute; Phillips Classic Laser & Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    2. Prokhorov General Physics Institute, Moscow 119991, Russia
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  • Jin-Woo Kim,

    1. Department of Biological and Agricultural Engineering and Institute for Nanoscale Materials Science and Engineering, University of Arkansas, Fayetteville, AR 72701, USA
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  • Valery V. Tuchin,

    1. Institute of Optics and Biophotonics, Saratov State University, Saratov 410012, Russia
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  • Vladimir P. Zharov

    1. Winthrop P. Rockefeller Cancer Institute; Phillips Classic Laser & Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
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Abstract

This report introduces a novel diagnostic and therapeutic platform for in vivo non-invasive detection and treatment of metastases in sentinel lymph nodes (SLNs) at single cell level using an integrated system of multicolor photoacoustic (PA) lymph flow cytometry, PA lymphography, absorption image cytometry, and photothermal (PT) therapy. A melanoma-bearing mouse model was used to demonstrate the capability of this platform for real-time lymphatic mapping, counting of disseminated tumor cells (DTCs) in prenodal lymphatics, and detecting metastasis in SLNs and its purging. The detection and ablation of non-pigmented breast cancer cells in SLNs was achieved by labeling them with nanoparticles. The association between DTC count and SLN metastasis progression supports lymphatic DTCs as a novel prognostic marker of metastasis. The fiber-based portable PA device may replace the conventional SLN(s) excision and histology-based staging. The earliest detection of DTCs in the lymphatic vessels before the establishment of nodal metastasis may prevent metastasis by well-timed ablation of DTCs. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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