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In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles

  1. Ekaterina I. Galanzha2,
  2. Mimi S. Kokoska3,
  3. Evgeny V. Shashkov1,4,
  4. Jin-Woo Kim5,
  5. Valery V. Tuchin2,
  6. Vladimir P. Zharov1

Article first published online: 9 SEP 2009

DOI: 10.1002/jbio.200910046

Issue

Journal of Biophotonics

Journal of Biophotonics

Special Issue: Towards in vivo Flow Cytometry

Volume 2, Issue 8-9, pages 528–539, September 2009

Additional Information

How to Cite

Galanzha, E. I., Kokoska, M. S., Shashkov, E. V., Kim, J.-W., Tuchin, V. V. and Zharov, V. P. (2009), In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles. Journal of Biophotonics, 2: 528–539. doi: 10.1002/jbio.200910046

Author Information

  1. 1

    Winthrop P. Rockefeller Cancer Institute; Phillips Classic Laser & Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA

  2. 2

    Institute of Optics and Biophotonics, Saratov State University, Saratov 410012, Russia

  3. 3

    Richard L. Roudebush VA Medical Center and Indiana University School of Medicine, Department of Otolaryngology-Head & Neck Surgery, Indianapolis, IN 46202, USA

  4. 4

    Prokhorov General Physics Institute, Moscow 119991, Russia

  5. 5

    Department of Biological and Agricultural Engineering and Institute for Nanoscale Materials Science and Engineering, University of Arkansas, Fayetteville, AR 72701, USA

Publication History

  1. Issue published online: 9 SEP 2009
  2. Article first published online: 9 SEP 2009
  3. Manuscript Revised: 17 AUG 2009
  4. Manuscript Accepted: 17 AUG 2009
  5. Manuscript Received: 12 JUN 2009

Funded by

  • National Institute of Health. Grant Numbers: R01 EB000873, R01 CA131164, R01 EB009230, R21 CA139373
  • National Science Foundation. Grant Number: DBI 0852737 (VPZ)
  • Arkansas Biosciences Institute (JWK and VPZ)

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Keywords:

  • metastasis;
  • sentinel lymph nodes;
  • photoacoustic imaging;
  • photothermal therapy;
  • nanoparticles

Abstract

This report introduces a novel diagnostic and therapeutic platform for in vivo non-invasive detection and treatment of metastases in sentinel lymph nodes (SLNs) at single cell level using an integrated system of multicolor photoacoustic (PA) lymph flow cytometry, PA lymphography, absorption image cytometry, and photothermal (PT) therapy. A melanoma-bearing mouse model was used to demonstrate the capability of this platform for real-time lymphatic mapping, counting of disseminated tumor cells (DTCs) in prenodal lymphatics, and detecting metastasis in SLNs and its purging. The detection and ablation of non-pigmented breast cancer cells in SLNs was achieved by labeling them with nanoparticles. The association between DTC count and SLN metastasis progression supports lymphatic DTCs as a novel prognostic marker of metastasis. The fiber-based portable PA device may replace the conventional SLN(s) excision and histology-based staging. The earliest detection of DTCs in the lymphatic vessels before the establishment of nodal metastasis may prevent metastasis by well-timed ablation of DTCs. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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