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Keywords:

  • medical and biological imaging;
  • spectroscopy;
  • coherent anti-Stokes Raman scattering;
  • nonlinear microscopy;
  • three-dimensional microscopy

Abstract

Studies of tissue remodeling require in vivo imaging techniques that are as minimally invasive as possible to avoid microenvironment perturbations. To this end, spontaneous Raman techniques have been used but low signals have limited their application mostly to point spectroscopy measurements. Novel Raman-based techniques such as coherent and stimulated Raman scattering can overcome this limitation. This manuscript discusses imaging and spectroscopy applications with Raman-based contrast for in vivo tissue monitoring, and how these can be combined into spectral imaging. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)