Application of Terahertz Time-Domain Spectroscopy in Intracellular Metabolite Detection

Authors

  • Changlei Wang,

    Corresponding author
    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
    • Phone: +86 22 27404204, Fax: +86 22 27404204
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  • Jixian Gong,

    1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, P.R. China
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  • Qirong Xing,

    Corresponding author
    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
    • Phone: +86 22 27404204, Fax: +86 22 27404204
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  • Yanfeng Li,

    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
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  • Feng Liu,

    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
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  • Xueming Zhao,

    1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, P.R. China
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  • Lu Chai,

    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
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  • Chingyue Wang,

    1. Centre for THz Waves, Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin 300072, P.R. China
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  • Aleksei M. Zheltikov

    1. Department of Physics, International laser Center, M. V. Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119992, Russia
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Abstract

Using terahertz time-domain spectroscopy (THz-TDS), we have investigated the THz spectra of astaxanthin and riboflavin and the spectra of two kinds of cell, haenatcoccus plusivalis and bacillus subtilis, which could produce astaxanthin and riboflavin, respectively, during their metabolite process. Riboflavin was found to be much more absorptive to THz radiation and have richer spectral characteristics than astaxanthin. As an intracellular metabolite, riboflavin could be distinguished from the cells by using THz-TDS. The technique has potential applications in high-throughput screening of industrial strains. (© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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