Multi-photon resonance enhancement of third harmonic generation in human oxyhemoglobin and deoxyhemoglobin

Authors

  • Chieh-Feng Chang,

    1. Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
    2. Currently with the Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, U.S.A.
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  • Che-Hang Yu,

    1. Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
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  • Chi-Kuang Sun

    Corresponding author
    1. Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
    2. Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan
    • Phone: +886 2 33665085, Fax: +886 2 33663614
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Abstract

Cancer cells require excessive oxygen and nutrition to support their rapid growth, so angiogenesis and decrease of blood oxygen are often associated with areas of cancer development. Current technologies for blood oxygen measurement, however, do not possess high spatial resolution and therefore cannot be used to detect small tumors in their early stage. In this paper, we studied the third harmonic generation (THG) spectra of oxy- and deoxyhemoglobin in the 1170–1365 nm region, which is strongly influenced by the multi-photon resonance effect, especially around the Soret transition band. Our spectroscopic results thus indicate the high potential of THG spectroscopic microscopy for oxygen depletion level measurement of a single red blood cell in vivo. (© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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