Letter

Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

  1. Gangjun Liu1,2,*,
  2. Khanh Kieu3,
  3. Frank W. Wise4,
  4. Zhongping Chen1,2,*

Article first published online: 16 JUL 2010

DOI: 10.1002/jbio.201000049

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 4, Issue 1-2, pages 34–39, January 2011

Additional Information

How to Cite

Liu, G., Kieu, K., Wise, F. W. and Chen, Z. (2011), Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe. J. Biophoton., 4: 34–39. doi: 10.1002/jbio.201000049

Author Information

  1. 1

    Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA

  2. 2

    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA

  3. 3

    College of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA

  4. 4

    Department of Applied Physics, Cornell University, Ithaca, NY 14853, USA

*Phone: +01 949 824 5597, Fax: +01 949 824 8413

Publication History

  1. Issue published online: 17 DEC 2010
  2. Article first published online: 16 JUL 2010
  3. Manuscript Accepted: 29 JUN 2010
  4. Manuscript Revised: 27 JUN 2010
  5. Manuscript Received: 1 APR 2010

Funded by

  • National Institutes of Health. Grant Numbers: EB-00293, CA-91717, EB-10090, RR-01192
  • Air Force Office of Scientific Research. Grant Number: FA9550-04-0101
  • Beckman Laser Institute Endowment

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Keywords:

  • multiphoton processes;
  • nonlinear microscopy;
  • hand-held probe;
  • fiber laser

Abstract

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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