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Keywords:

  • selective plane illumination microscopy;
  • SPIM;
  • cell tracking;
  • zebrafish;
  • lateral line

Abstract

A study demonstrating an imaging framework that permits the determination of cell lineages during organogenesis of the posterior lateral line in zebrafish is presented. The combination of Selective Plane Illumination Microscopy and specific fluorescent markers allows retrospective tracking of hair cell progenitors, and hence the derivation of their lineages within the primodium. It is shown that, because of its superior signal-to-noise ratio and lower photo-damaged properties, SPIM can provide significantly higher-quality images than Spinning Disk Confocal technology. This allows accurate 4D lineage tracing for the hair cells over tens of hours of primordium migration and neuromast development. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)