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Keywords:

  • label-free imaging;
  • particle tracking;
  • CARS microscopy;
  • lipid droplets

Abstract

Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label-free imaging techniques therefore offer advantages both for rapid image acquisition and for long–term observations. CARS microscopy is a label-free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video-rate tracking of intracellular transport of lipid droplets, but also continuous long-term observation of cells over several hours. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)