Raman spectroscopy for the non-contact and non-destructive monitoring of collagen damage within tissues

Authors

  • Miriam Votteler,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, 70569 Stuttgart, Germany
    2. Inter-University Centre for Medical Technology Stuttgart-Tübingen IZST, Eberhard Karls University Tübingen, Germany
    3. Dept. of Thoracic, Cardiac and Vascular Surgery, University Hospital UKT, Eberhard Karls University Tübingen, Germany
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    • Both authors contributed equally to this work.

  • Daniel A. Carvajal Berrio,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, 70569 Stuttgart, Germany
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    • Both authors contributed equally to this work.

  • Marieke Pudlas,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, 70569 Stuttgart, Germany
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  • Heike Walles,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, 70569 Stuttgart, Germany
    2. Dept. of Tissue Engineering and Regenerative Medicine, University of Würzburg, Germany
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  • Ulrich A. Stock,

    1. Dept. of Thoracic, Cardiac and Vascular Surgery, University Hospital UKT, Eberhard Karls University Tübingen, Germany
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  • Katja Schenke-Layland

    Corresponding author
    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, 70569 Stuttgart, Germany
    2. Inter-University Centre for Medical Technology Stuttgart-Tübingen IZST, Eberhard Karls University Tübingen, Germany
    3. Dept. of Thoracic, Cardiac and Vascular Surgery, University Hospital UKT, Eberhard Karls University Tübingen, Germany
    • Phone: +49 711/970-4082, Fax: +49 711/970-4158
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Abstract

The non-destructive and label-free monitoring of extracellular matrix (ECM) remodeling and degradation processes is a great challenge. Raman spectroscopy is a non-contact method that offers the possibility to analyze ECM in situ without the need for tissue processing. Here, we employed Raman spectroscopy for the detection of heart valve ECM, focusing on collagen fibers. We screened the leaflets of porcine aortic valves either directly after dissection or after treatment with collagenase. By comparing the fingerprint region of the Raman spectra of control and treated tissues (400–1800 cm–1), we detected no significant differences based on Raman shifts; however, we found that increasing collagen degradation translated into decreasing Raman signal intensities. After these proof-of-principal experiments, we compared Raman spectra of native and cryopreserved valve tissues and revealed that the signal intensities of the frozen samples were significantly lower compared to those of native tissues, similar to the data seen in the enzymatically-degraded tissues. In conclusion, our data demonstrate that Raman microscopy is a promising, non-destructive and non-contact tool to probe ECM state in situ. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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