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Keywords:

  • mitochondria;
  • cytochrome redox state;
  • isolation;
  • respiration activity;
  • resonance Raman scattering

Abstract

We observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)