Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry

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Abstract

We use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess-light conditions on the physiological state of the green alga Chlorella pyrenoidosa. We demonstrate that zeaxanthin-dependent non-photochemical quenching leads to a significant increase in the rate constant of singlet-singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light-harvesting complexes of photosystem II. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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