Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry



We use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess-light conditions on the physiological state of the green alga Chlorella pyrenoidosa. We demonstrate that zeaxanthin-dependent non-photochemical quenching leads to a significant increase in the rate constant of singlet-singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light-harvesting complexes of photosystem II. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)