In vivo imaging of hepatic excretory function in the rat by fluorescence microscopy

Authors

  • Peter Recknagel,

    1. Integrated Research and Treatment Center – Center for Sepsis Control and Care CSCC, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
    2. Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Jena, Germany
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  • Ralf A. Claus,

    1. Integrated Research and Treatment Center – Center for Sepsis Control and Care CSCC, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
    2. Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Jena, Germany
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  • Ute Neugebauer,

    1. Integrated Research and Treatment Center – Center for Sepsis Control and Care CSCC, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
    2. Institute of Photonic Technology IPHT, Jena, Germany
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  • Michael Bauer,

    Corresponding author
    1. Integrated Research and Treatment Center – Center for Sepsis Control and Care CSCC, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
    2. Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Jena, Germany
    • Phone: ++49 3641 9323101, Fax: ++49 3641 9323102
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  • Falk A. Gonnert

    1. Integrated Research and Treatment Center – Center for Sepsis Control and Care CSCC, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
    2. Department of Anaesthesiology and Intensive Care Therapy, Jena University Hospital, Jena, Germany
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Abstract

Applying intravital fluorescence microscopy, we assessed sinusoidal delivery and biliary clearance of two different polymethine dyes. DY635, a benzopyrylium-based hemocyanine dye with shorter excitation wavelength than indocyanine green (ICG), was validated for assessment of hepatic excretory function. Decrease of DY635 and ICG reflecting transcellular transport was 83 ± 4% (DY635) and 14 ± 2% (ICG; p < 0.05) over 35 minutes, respectively. In cholestasis, hepatobiliary excretion of DY635 was markedly impaired (control 3176 ± 148 pmol vs. cholestatic 1929 ± 179 pmol; p < 0.05). DY635 even enabled an analysis at high resolution suggesting 1.) hepatocyte uncoupling and 2.) failure of primarily the canalicular pole, allowing in vivo insights into molecular mechanisms of this critical facet of hepatobiliary function. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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