CRAFT: Multimodality confocal skin imaging for early cancer diagnosis

Authors

  • Tong Peng,

    1. Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
    2. Department of Physics, Xi'an University of Technology, No. 5 South Jinhua Road, Xi'an 710048, China
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  • Hao Xie,

    1. Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
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  • Yichen Ding,

    1. Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
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  • Weichao Wang,

    1. Department of Biomedical Engineering, College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China
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  • Zhiming Li,

    1. Department of Dermatology and Venereology, the First Affiliated Hospital of Wenzhou Medical College, No. 2 Fuxue Road, Wenzhou, Zhejiang 325000, China
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  • Dayong Jin,

    1. Advanced Cytometry Labs, MQphotonics Research Centre, Macquarie University, NSW 2109, Sydney, Australia
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  • Yuanhe Tang,

    1. Department of Physics, Xi'an University of Technology, No. 5 South Jinhua Road, Xi'an 710048, China
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  • Qiushi Ren,

    1. Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
    2. Department of Biomedical Engineering, College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China
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  • Peng Xi

    Corresponding author
    1. Department of Biomedical Engineering, College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
    • Phone: +86 10 6276 7155, Fax: +86 10 6276 7155
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Abstract

Although histological analysis serves as a gold standard to cancer diagnosis, its application on skin cancer detection is largely prohibited due to its invasive nature. To obtain both the structural and pathological information in situ, a Confocal Reflectance/Auto-Fluorescence Tomography (CRAFT) system was established to examine the skin sites in vivo with both reflectance and autofluorescence modes simultaneously. Nude mice skin with cancerous sites and normal skin sites were imaged and compared with the system. The cellular density and reflective intensity in cancerous sites reflects the structural change of the tissue. With the decay coefficient analysis, the corresponding NAD(P)H decay index for cancerous sites is 1.65-fold that of normal sites, leading to a 97.8% of sensitivity and specificity for early cancer diagnosis. The results are verified by the followed histological analysis. Therefore, CRAFT may provide a novel method for the in vivo, non-invasive diagnosis of early cancer. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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