Editor's Choice

You have free access to this content

In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas

  1. Alex J. Thompson1,*,
  2. Sergio Coda1,6,
  3. Mikkel Brydegaard Sørensen2,
  4. Gordon Kennedy1,
  5. Rakesh Patalay1,3,
  6. Ulrika Waitong-Brämming4,
  7. Pieter A. A. De Beule1,
  8. Mark A. A. Neil1,
  9. Stefan Andersson-Engels2,
  10. Niels Bendsøe5,
  11. Paul M. W. French1,
  12. Katarina Svanberg4,
  13. Chris Dunsby1,*

Article first published online: 6 FEB 2012

DOI: 10.1002/jbio.201100126

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 5, Issue 3, pages 240–254, March 2012

Additional Information

How to Cite

Thompson, A. J., Coda, S., Sørensen, M. B., Kennedy, G., Patalay, R., Waitong-Brämming, U., De Beule, P. A. A., Neil, M. A. A., Andersson-Engels, S., Bendsøe, N., French, P. M. W., Svanberg, K. and Dunsby, C. (2012), In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas. J. Biophoton., 5: 240–254. doi: 10.1002/jbio.201100126

Author Information

  1. 1

    Photonics Group, Department of Physics, Imperial College, Prince Consort Road, London, UK

  2. 2

    Atomic Physics Division, Lund University, Lund, Sweden

  3. 3

    Department of Dermatology, Imperial College Healthcare NHS Trust, London, UK

  4. 4

    Department of Oncology, Lund University Hospital, Lund, Sweden

  5. 5

    Department of Dermatology, Lund University Hospital, Lund, Sweden

  6. 6

    Department of Gastroenterology, Imperial College Healthcare NHS Trust, London, UK

*Phone: +44 207 594 1278, Fax: +44 (0)20 7594 7714

Publication History

  1. Issue published online: 5 MAR 2012
  2. Article first published online: 6 FEB 2012
  3. Manuscript Accepted: 12 JAN 2012
  4. Manuscript Revised: 23 DEC 2011
  5. Manuscript Received: 18 NOV 2011

Funded by

  • Engineering and Physical Sciences Research Council (EPSRC EP/F040202/1)
  • Photonics-4-Life European Network (FP7-ICT-2007-2, 224014)
  • SKINSPECTION project (FP7-HEALTH-2007-A, 201577)
  • EPSRC funded Ph.D. studentship
  • PIEp
  • Swedish innovation initiative
  • Royal Society Wolfson Research Merit Award

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (451K)

Keywords:

  • fluorescence;
  • fluorescence lifetime;
  • diffuse reflectance;
  • skin cancer;
  • spectrometer;
  • fibre probe

Abstract

We present a clinical investigation of diffuse reflectance and time-resolved autofluorescence spectra of skin cancer with an emphasis on basal cell carcinoma. A total of 25 patients were measured using a compact steady-state diffuse reflectance/fluorescence spectrometer and a fibre-optic-coupled multispectral time-resolved spectrofluorometer. Measurements were performed in vivo prior to surgical excision of the investigated region. Singular value decomposition was used to reduce the dimensionality of steady state diffuse reflectance and fluorescence spectra. Linear discriminant analysis was then applied to the measurements of basal cell carcinomas (BCCs) and used to predict the tissue disease state with a leave-one-out methodology. This approach was able to correctly diagnose 87% of the BCCs. With 445 nm excitation a decrease in the spectrally averaged fluorescence lifetime was observed between normal tissue and BCC lesions with a mean value of 886 ps. Furthermore, the fluorescence lifetime for BCCs was lower than that of the surrounding healthy tissue in all cases and statistical analysis of the data revealed that this decrease was significant (p = 0.002). (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Get PDF (451K)