Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems

Authors

  • Erich E. Hoover,

    Corresponding author
    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
    • Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • Jeffrey J. Field,

    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • David G. Winters,

    1. Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA
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  • Michael D. Young,

    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • Eric V. Chandler,

    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • John C. Speirs,

    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • Jacob T. Lapenna,

    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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  • Susy M. Kim,

    1. Section for Neurobiology, Division of Biological Sciences, University of California-San Diego, 9500 Gilman Drive, MC 0368, La Jolla, CA 92093-0368, USA
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  • Shi-you Ding,

    1. National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, Colorado 80401, USA
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  • Randy A. Bartels,

    1. Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA
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  • Jing W. Wang,

    1. Section for Neurobiology, Division of Biological Sciences, University of California-San Diego, 9500 Gilman Drive, MC 0368, La Jolla, CA 92093-0368, USA
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  • Jeff A. Squier

    Corresponding author
    1. Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
    • Center for Microintegrated Optics for Advanced Bioimaging and Control, and Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA
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Abstract

In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire volumes in the time it takes to perform a single transverse scan (four depths simultaneously at a rate of 30 Hz). In addition, this capability is further exploited to accomplish single-element detection of multiple modalities (two photon excited fluorescence and second harmonic generation) and to perform efficient image deconvolution. Finally, we demonstrate a new system that promises to significantly simplify this promising technology. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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