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Keywords:

  • laser trapping;
  • laser ablation;
  • optical micromanipulation;
  • robotic laser microscope system

Abstract

A multi-joystick robotic laser microscope system used to control two optical traps (tweezers) and one laser scissors has been developed for subcellular organelle manipulation. The use of joysticks has provided a “user-friendly” method for both trapping and cutting of organelles such as chromosomes in live cells. This innovative design has enabled the clean severing of chromosome arms using the laser scissors as well as the ability to easily hold and pull the severed arm using the laser tweezers. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)