Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy

Authors

  • Minoru Kakita,

    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Toyo 113-0033, Japan
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  • Masanari Okuno,

    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Toyo 113-0033, Japan
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  • Hiro-o Hamaguchi

    Corresponding author
    1. Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Toyo 113-0033, Japan
    2. Institute of Molecular Science and Department of Applied Chemistry, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300, Taiwan
    • Phone: +81 3 5841 4329, Fax: +81 3 3818 4621
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Abstract

Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c, while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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