Nanofluidic devices towards single DNA molecule sequence mapping

Authors

  • Rodolphe Marie,

    Corresponding author
    1. Department of micro- and nanotechnology, Technical University of Denmark, Oersteds plads Building 345east, 2800 Kongens Lyngby, Denmark
    • Department of micro- and nanotechnology, Technical University of Denmark, Oersteds plads Building 345east, 2800 Kongens Lyngby, Denmark
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  • Anders Kristensen

    1. Department of micro- and nanotechnology, Technical University of Denmark, Oersteds plads Building 345east, 2800 Kongens Lyngby, Denmark
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Abstract

Nanofluidics enables the imaging of stretched single molecules with potential applications for single molecule sequence mapping. Lab-on-a-chip devices for single cell trapping and lyzing, genomic DNA extraction from single cells, and optical mapping of genomic length DNA has been demonstrated separately. Yet the pursuit for applying DNA optical mapping to solve real genomics challenges is still to come. We review lab-on-a-chip devices from literature that could be part of a complete system for the sequence mapping of single DNA molecules. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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