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A quantitative bioapatite/collagen calibration method using Raman spectroscopy of bone

  1. I. A. Karampas1,2,
  2. M. G. Orkoula1,2,
  3. C. G. Kontoyannis1,2,*

Article first published online: 7 SEP 2012

DOI: 10.1002/jbio.201200053

Additional Information

How to Cite

Karampas, I. A., Orkoula, M. G. and Kontoyannis, C. G. (2012), A quantitative bioapatite/collagen calibration method using Raman spectroscopy of bone. J. Biophoton.. doi: 10.1002/jbio.201200053

Author Information

  1. 1

    Department of Pharmacy, University of Patras, 26500 Patras, Greece

  2. 2

    Institute of Chemical Engineering and High Temperature Chemical Processes, FORTH, Patras, Greece

*Phone: +30 2610 969328, Fax: +30 2610 997658

Publication History

  1. Article first published online: 7 SEP 2012
  2. Manuscript Accepted: 9 AUG 2012
  3. Manuscript Revised: 8 AUG 2012
  4. Manuscript Received: 21 MAR 2012

Funded by

  • Research Committee of University of Patras (Karatheodoris program)

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Keywords:

  • collagen;
  • bone mineral;
  • proline;
  • amide I;
  • hydroxyproline;
  • quantitative analysis;
  • bioapatite;
  • Raman spectroscopy

Abstract

Numerous calibration models were developed and tested for the quantitative analysis of collagen and bioapatite in bone using Raman spectroscopy. The ν1 phosphate vibration at 960 cm–1 was used as indicator of the content of bioapatite while for collagen three markers were used: the C–H2 band at 2940 cm–1, the amide I band at 1667 cm–1 and the vibrations of proline and hydroxyproline at 855 and 878 cm–1, respectively. Also a calibration model based on the PLS algorithm was developed, too. Validation of the derived calibration models indicated that the model that makes use of the height ratio of the peaks 960/(855+878) exhibits the best accuracy. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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