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Keywords:

  • Cancer;
  • radiation risk;
  • noninvasive detection;
  • Raman spectroscopy

Abstract

Using the AKR/J mouse model, the potential of Raman spectroscopy for monitoring lymphoma in predisposed subjects is demonstrated by discriminating lymphoma infiltration in spleens; the relevance of different excitation profiles is shown. Under green excitation with optimal fluorescence bleaching, stronger DNA bands, intensity variations at amide-III and phenylalanine bands, and the behavior of the 1606/1639 cm–1 doublet correlate with tumorigenesis. Under red excitation, Raman fingerprints with multivariate models help to discriminate AKR/J-mouse histological subtypes: Lymphoblastic lymphoma (LB) is found significantly distant from both separated lymphocytic lymphoma (LL) and healthy spleen; this agrees with histology since LB has well differentiated large lymphoma cells, while LL, with smaller cells similar to normal lymphocytes, usually cannot be discriminated from normal tissue without histoimmunoassays. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)