Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy

Authors

  • Marieke Pudlas,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, Germany
    2. University of Stuttgart, Institute for Interfacial Engineering IGVT, Nobelstr. 12, 70569 Stuttgart, Germany
    3. Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, 4059, Australia
    Search for more papers by this author
    • Both authors contributed equally to this work.

  • Eva Brauchle,

    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, Germany
    2. Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen IZST at the University Hospital Tübingen, Eberhard Karls University Tübingen, Silcherstr. 7, 72076 Tübingen, Germany
    Search for more papers by this author
    • Both authors contributed equally to this work.

  • Travis J. Klein,

    1. Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, 4059, Australia
    Search for more papers by this author
  • Dietmar W. Hutmacher,

    1. Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, 4059, Australia
    2. George W. Woodruff School of Mechanical Engineering, 315 Ferst Drive, Georgia Institute of Technology, Atlanta, GA 30318, USA
    Search for more papers by this author
  • Katja Schenke-Layland

    Corresponding author
    1. Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Dept. of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, Germany
    2. Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen IZST at the University Hospital Tübingen, Eberhard Karls University Tübingen, Silcherstr. 7, 72076 Tübingen, Germany
    • Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Dept. of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, Germany
    Search for more papers by this author

Abstract

Proteoglycans (PGs) are crucial extracellular matrix (ECM) components that are present in all tissues and organs. Pathological remodeling of these macromolecules can lead to severe diseases such as osteoarthritis or rheumatoid arthritis. To date, PG-associated ECM alterations are routinely diagnosed by invasive analytical methods. Here, we employed Raman microspectroscopy, a laser-based, marker-free and non-destructive technique that allows the generation of spectra with peaks originating from molecular vibrations within a sample, to identify specific Raman bands that can be assigned to PGs within human and porcine cartilage samples and chondrocytes. Based on the non-invasively acquired Raman spectra, we further revealed that a prolonged in vitro culture leads to phenotypic alterations of chondrocytes, resulting in a decreased PG synthesis rate and loss of lipid contents. Our results are the first to demonstrate the applicability of Raman microspectroscopy as an analytical and potential diagnostic tool for non-invasive cell and tissue state monitoring of cartilage in biomedical research. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Ancillary