Impact of cell division on intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles

Authors

  • Tetiana Serdiuk,

    Corresponding author
    1. University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France
    2. University of Lyon, Nanotechnology Institute of Lyon INL, UMR CNRS 5270, INSA de Lyon, France
    3. Institute of High Technologies, Taras Shevchenko National University of Kyiv 64, Volodymyrs'ka St., 01601 Kyiv, Ukraine
    • University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France
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  • Vladimir Lysenko,

    1. University of Lyon, Nanotechnology Institute of Lyon INL, UMR CNRS 5270, INSA de Lyon, France
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  • Barbara Mognetti,

    1. Dpt. of Clinical and Biological Sciences, University of Turin, AOU S. Luigi, 10043 Orbassano, Turin, Italy
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  • Valery Skryshevsky,

    1. Institute of High Technologies, Taras Shevchenko National University of Kyiv 64, Volodymyrs'ka St., 01601 Kyiv, Ukraine
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  • Alain Géloën

    Corresponding author
    1. University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France
    • University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France
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Abstract

Semiconductor nanoparticles (NPs) became important and wide-used tool for cell imaging because of their unique remarkable properties. Nevertheless, all previous investigations in this area were done on proliferating cells. For the first time, this work demonstrates strong influence of cell active proliferation/contact inhibition of proliferation on uptake of NPs. In addition, we show that cell division plays key-role in penetration of silicon carbide based NPs (SiC NPs) inside the cell nucleus. This may very likely concern other types of NPs able to reach the cell nuclei. In particular, observed effect of cell division gives perspectives for future selective cancer treatment with NPs. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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