Perfectly registered multiphoton and reflectance confocal video rate imaging of in vivo human skin

Authors

  • Hequn Wang,

    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    Search for more papers by this author
    • These authors contributed equally to this work

  • Anthony M.D. Lee,

    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    2. Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada
    Search for more papers by this author
    • These authors contributed equally to this work

  • Zack Frehlick,

    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    Search for more papers by this author
  • Harvey Lui,

    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    2. Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, Vancouver, BC, Canada
    Search for more papers by this author
  • David I. McLean,

    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    2. Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, Vancouver, BC, Canada
    Search for more papers by this author
  • Shuo Tang,

    1. Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC, Canada
    Search for more papers by this author
  • Haishan Zeng

    Corresponding author
    1. Imaging Unit – Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada
    2. Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada
    3. Photomedicine Institute, Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, Vancouver, BC, Canada
    • Phone: +1 604 675 8083, Fax: +1 604 675 8099
    Search for more papers by this author

Abstract

We present a multimodal in vivo skin imaging instrument that is capable of simultaneously acquiring multiphoton and reflectance confocal images at up to 27 frames per second with 256 × 256 pixel resolution without the use of exogenous contrast agents. A single femtosecond laser excitation source is used for all channels ensuring perfect image registration between the two-photon fluorescence (TPF), second harmonic generation (SHG), and reflectance confocal microscopy (RCM) images. Images and videos acquired with the system show that the three imaging channels provide complementary information in in vivo human skin measurements. In the epidermis, cell boundaries are clearly seen in the RCM channel, while cytoplasm is better seen in the TPF imaging channel, whereas in the dermis, SHG and TPF channels show collagen bundles and elastin fibers, respectively. The demonstrated fast imaging speed and multimodal imaging capabilities of this MPM/RCM instrument are essential features for future clinical application of this technique. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Ancillary