Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy

Authors

  • Clara Sousa,

    1. REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
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  • Filipa Grosso,

    1. REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
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  • Luís Meirinhos-Soares,

    1. Direcção de Comprovação da Qualidade do INFARMED I.P., Parque de Saúde de Lisboa, Avenida do Brasil, n° 53, 1749-004 Lisboa, Portugal
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  • Luísa Peixe,

    1. REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
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  • João Lopes

    Corresponding author
    1. REQUIMTE, Laboratório de Química Analítica e Físico-Química, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
    • Phone: +351 220428664, Fax: +351 226093483===

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Abstract

In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D β -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001–2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58, ST98 carrying blaOXA-24/40and ST92 carrying blaOXA-23. Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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