Measuring anisotropic cell motility on curved substrates

Authors

  • Kyle M. Douglass,

    1. CREOL, The College of Optics and Photonics, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA
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  • Nicklaus A. Sparrow,

    1. Department of Molecular Biology and Microbiology, Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Health Science Campus, 6900 Lake Nona Boulevard, Orlando, FL, 32827, USA
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  • Marga Bott,

    1. Department of Molecular Biology and Microbiology, Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Health Science Campus, 6900 Lake Nona Boulevard, Orlando, FL, 32827, USA
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  • Cristina Fernandez-Valle,

    1. Department of Molecular Biology and Microbiology, Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Health Science Campus, 6900 Lake Nona Boulevard, Orlando, FL, 32827, USA
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  • Aristide Dogariu

    Corresponding author
    1. CREOL, The College of Optics and Photonics, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA
    • CREOL, The College of Optics and Photonics, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816,USA
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Abstract

Schwann cell motility was observed on laminin-coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 μm, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 μm per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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