• Please log in or register to access this feature.

Editor's Choice

You have free access to this content

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

  1. Riccardo Cicchi1,2,*,
  2. Nadine Vogler3,4,
  3. Dimitrios Kapsokalyvas2,
  4. Benjamin Dietzek3,4,
  5. Jürgen Popp3,4,
  6. Francesco Saverio Pavone1,5

Article first published online: 12 JUL 2012

DOI: 10.1002/jbio.201200092

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 6, Issue 2, pages 129–142, February 2013

Additional Information

How to Cite

Cicchi, R., Vogler, N., Kapsokalyvas, D., Dietzek, B., Popp, J. and Pavone, F. S. (2013), From molecular structure to tissue architecture: collagen organization probed by SHG microscopy. J. Biophoton., 6: 129–142. doi: 10.1002/jbio.201200092

Author Information

  1. 1

    European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy

  2. 2

    National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125 Florence, Italy

  3. 3

    Institute of Photonic Technology Jena IPHT, Albert Einstein Straße 9, 07745 Jena, Germany

  4. 4

    Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University, Helmholtzweg 4, 07743 Jena, Germany

  5. 5

    Department of Physics, University of Florence, Via G. Sansone 1, 50019 Sesto Fiorentino, Italy

*Phone: +39 055 457 2476, Fax: +39 055 457 2451

Publication History

  1. Issue published online: 1 FEB 2013
  2. Article first published online: 12 JUL 2012
  3. Manuscript Revised: 15 JUN 2012
  4. Manuscript Accepted: 15 JUN 2012
  5. Manuscript Received: 18 MAY 2012

Funded by

  • European Union Seventh Framework Programme (FP7/2007–2013). Grant Number: 228334
  • Italian Ministry for Education, University and Research in the framework of the Flagship Project NANOMAX
  • German Federal Ministry of Science and Education (project MediCARS). Grant Number: 13N10774
  • Ente Cassa di Risparmio di Firenze (private foundation)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (439K)

Keywords:

  • second-harmonic generation (SHG) microscopy;
  • tissue imaging;
  • collagen

Abstract

Second-harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non-linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward – backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Get PDF (439K)