Multiphoton imaging of freezing and heating effects in plant leaves

Authors

  • Hans Georg Breunig,

    Corresponding author
    1. JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany
    • JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany
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  • Fatma Tümer,

    1. JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany
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  • Karsten König

    1. JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany
    2. Department of Biophotonics and Laser Technology, Saarland University, Campus A 5.1, 66123 Saarbrücken, Germany
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Abstract

Thermally-induced changes in Arabidopsis thaliana leaves were investigated with a novel cryo microscope by multiphoton, fluorescence lifetime and spectral imaging as well as micro spectroscopy. Samples were excited with fs pulses in the near-infrared range and cooled/heated in a cryogenic chamber. The results show morphological changes in the chloroplast distribution as well as a shift from chlorophyll to cell-wall fluorescence with decreasing temperature. At temperatures below –40 °C, also second harmonic generation was observed. The measurements illustrate the suitability of multiphoton imaging to investigate thermally-induced changes at temperatures used for cryopreservation as well as for basic investigations of thermal effects on plant tissue in general (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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