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Keywords:

  • breast cancer;
  • optical coherence tomography;
  • polarization;
  • imaging;
  • fluorescence;
  • reflectance;
  • contrast agents

Abstract

Intra-operative delineation of breast cancer is a challenging problem. We used dye-enhanced wide-field polarization imaging for rapid demarcation of en face cancer margins and optical coherence tomography (OCT) for cross-sectional evaluation. Ductal carcinoma specimens were stained with methylene blue. Wide-field reflectance images were acquired at 440 and 640 nm. Wide-field fluorescence images were excited at 640 nm and registered between 660 nm and 750 nm. OCT images were acquired using a 1310 nm swept-source system. The results were validated against histopathology. Both imaging modalities provided diagnostic information on cancer margins. Combined OCT and wide-field polarization imaging shows promise for intra-operative detection of ductal breast carcinoma (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)