Second harmonic generation microscopy as a powerful diagnostic imaging modality for human ovarian cancer

Authors

  • Javier Adur,

    Corresponding author
    1. Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, “Gleb Wataghin” Institute of Physics, State University of Campinas UNICAMP, Brazil
    2. Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Bioengineering, National University of Entre Ríos UNER, Ruta 11 Km10, Oro Verde 3101, Entre Ríos, Argentina
    • Phone: 0054-343-4975100

    Search for more papers by this author
  • Vitor B. Pelegati,

    1. Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, “Gleb Wataghin” Institute of Physics, State University of Campinas UNICAMP, Brazil
    Search for more papers by this author
  • Andre A. de Thomaz,

    1. Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, “Gleb Wataghin” Institute of Physics, State University of Campinas UNICAMP, Brazil
    Search for more papers by this author
  • Mariana O. Baratti,

    1. INFABiC, National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
    Search for more papers by this author
  • Liliana A. L. A. Andrade,

    1. Department of Pathology of the Faculty of Medical Sciences, State University of Campinas UNICAMP, Brazil
    Search for more papers by this author
  • Hernandes F. Carvalho,

    1. INFABiC, National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
    2. Department of Anatomy, Cell Biology, Physiology and Biophysics, Biology Institute, State University of Campinas UNICAMP, Brazil
    Search for more papers by this author
  • Fátima Bottcher-Luiz,

    1. INFABiC, National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
    2. Department of Obstetrics and Gynecology of the Faculty of Medical Sciences, State University of Campinas UNICAMP, Brazil
    Search for more papers by this author
  • Carlos Lenz Cesar

    1. Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, “Gleb Wataghin” Institute of Physics, State University of Campinas UNICAMP, Brazil
    2. INFABiC, National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil
    Search for more papers by this author

Abstract

In this study we showed that second-harmonic generation (SHG) microscopy combined with precise methods for images evaluation can be used to detect structural changes in the human ovarian stroma. Using a set of scoring methods (alignment of collagen fibers, anisotropy, and correlation), we found significant differences in the distribution and organization of collagen fibers in the stroma component of serous, mucinous, endometrioid and mixed ovarian tumors as compared with normal ovary tissue. This methodology was capable to differentiate between cancerous and healthy tissue, with clear cut distinction between normal, benign, borderline, and malignant tumors of serous type. Our results indicated that the combination of different image-analysis approaches presented here represent a powerful tool to investigate collagen organization and extracellular matrix remodeling in ovarian tumors (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Ancillary