Discrimination of single-porin Escherichia (E.) coli mutants by ATR and transmission mode FTIR spectroscopy

Authors

  • Raúl G. Saraiva,

    1. REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, R. do Campo Alegre, 4169-007 Porto, Portugal
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  • João Almeida Lopes,

    1. REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
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  • Jorge Machado,

    1. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal
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  • Paula Gameiro,

    1. REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, R. do Campo Alegre, 4169-007 Porto, Portugal
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  • Maria J. Feio

    Corresponding author
    1. REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, R. do Campo Alegre, 4169-007 Porto, Portugal
    • Phone: +351 22 0402585, Fax: +351 22 0402659===

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Abstract

Vibrational spectroscopy has long been used in bacterial identification with different levels of taxonomic discrimination but its true potential for intra-species differentiation remains poorly explored. Herein, both transmission Fourier-transform infrared (FTIR) and attenuated total reflectance (ATR)-FTIR spectroscopy are used to analyse E. coli strains that differ solely in their porin expression profile. In this previously unreported approach, the applicability of both FTIR-spectroscopy techniques is compared with the same collection of unique strains. ATR-FTIR spectroscopy proved to reliably distinguish between several E. coli porin mutants with an accuracy not replicated by FTIR in transmission mode (using previously optimized procedures). Further studies should allow the identification of the individual contribution of the single porin channel to the overall bacterial infrared spectrum and of molecular predictive patterns of porin alterations. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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