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Recent advances in laser tweezers Raman spectroscopy (LTRS) for label-free analysis of single cells

  1. James W. Chan1,2,*

Article first published online: 23 NOV 2012

DOI: 10.1002/jbio.201200143

Issue

Journal of Biophotonics

Journal of Biophotonics

Special Issue: Vibrational Spectroscopy in Medicine

Volume 6, Issue 1, pages 36–48, January 2013

Additional Information

How to Cite

Chan, J. W. (2013), Recent advances in laser tweezers Raman spectroscopy (LTRS) for label-free analysis of single cells. J. Biophoton., 6: 36–48. doi: 10.1002/jbio.201200143

Author Information

  1. 1

    Department of Pathology and Laboratory Medicine, University of California, Davis, Sacramento, CA 95817, USA

  2. 2

    NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA 95817, USA

*Phone: (916) 734-0774, Fax: (916) 703-5012

Publication History

  1. Issue published online: 3 JAN 2013
  2. Article first published online: 23 NOV 2012
  3. Manuscript Accepted: 28 OCT 2012
  4. Manuscript Revised: 15 OCT 2012
  5. Manuscript Received: 24 JUL 2012

Funded by

  • National Science Foundation including the Center for Biophotonics
  • NSF Science and Technology Center Cooperative Agreement No. PHY 0120999
  • Center for Biophotonics Sensors and Systems award IIP1068109
  • Creation of an Ecosystem for Biophotonics Innovation award IIP1127888 managed by the University of California, Davis
  • Keaton Raphael Memorial Foundation

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Keywords:

  • Raman spectroscopy;
  • laser tweezers;
  • optical trapping;
  • cytometry;
  • label-free

Abstract

Laser tweezers Raman spectroscopy (LTRS), a technique that integrates optical tweezers with confocal Raman spectroscopy, is a variation of micro-Raman spectroscopy that enables the manipulation and biochemical analysis of single biological particles in suspension. This article provides an overview of the LTRS method, with an emphasis on highlighting recent advances over the past several years in the development of the technology and several new biological and biomedical applications that have been demonstrated. A perspective on the future developments of this powerful cytometric technology will also be presented. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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