Characterization of atherosclerotic plaque depositions by Raman and FTIR imaging

Authors

  • Annika Lattermann,

    1. University Hospital – Friedrich-Schiller-University of Jena, Clinic for Internal Medicine I Erlanger Allee 101, 07747 Jena, Germany
    2. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Christian Matthäus,

    1. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Norbert Bergner,

    1. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Claudia Beleites,

    1. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Bernd F. Romeike,

    1. Department of Neuropathology, University Hospital, Friedrich-Schiller University, Erlanger Allee 101, 07747 Jena, Germany
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  • Christoph Krafft,

    Corresponding author
    1. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
    • Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Bernhard R. Brehm,

    1. University Hospital – Friedrich-Schiller-University of Jena, Clinic for Internal Medicine I Erlanger Allee 101, 07747 Jena, Germany
    2. Present address: Catholic Clinic Koblenz, Department of Internal Medicine, Rudolf-Virchowstr. 9, 56078 Koblenz, Germany
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  • Jürgen Popp

    1. Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany
    2. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University of Jena, Helmholtzweg 4, 07743 Jena, Germany
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Abstract

Spectroscopy-based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima-media and the lipid-protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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