An in vitro study of the photodynamic effect of rose bengal on trichophyton rubrum

Authors

  • Leah Cronin,

    1. School of Science and Health, University of Western Sydney, Campbelltown NSW 2560, Australia
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  • Michelle Moffitt,

    1. School of Science and Health, University of Western Sydney, Campbelltown NSW 2560, Australia
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  • Damia Mawad,

    1. The Bioelectronics and Neuroscience BENS Research Group, University of Western Sydney, Campbelltown NSW 2560, Australia
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  • Oliver C. Morton,

    1. School of Science and Health, University of Western Sydney, Campbelltown NSW 2560, Australia
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  • Antonio Lauto,

    Corresponding author
    1. The Bioelectronics and Neuroscience BENS Research Group, University of Western Sydney, Campbelltown NSW 2560, Australia
    2. School of Medicine, University of Western Sydney, Campbelltown NSW 2560, Australia
    • Phone: +61 2 4620 3235, Fax: +61 2 4620 3025===

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    • Both authors contributed equally to the present publication.

  • Colin Stack

    1. School of Science and Health, University of Western Sydney, Campbelltown NSW 2560, Australia
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    • Both authors contributed equally to the present publication.


Abstract

Onychomycosis, a fungal infection of the finger or toenails, is predominantly caused by Trichophyton rubrum. Treatment is difficult due to high recurrence rates and problems with treatment compliance. For these reasons, alternative therapies are needed. Here we describe the photoactivation of Rose Bengal (RB) using a green laser (λ = 532 nm) at fluences of 68, 133 and 228 J/cm2, and assess its fungicidal activity on T. rubrum spore suspensions. A 140 µM RB solution was able to induce a fungicidal effect on T. rubrum when photosensitized with the fluence of 228 J/cm2. RB photosensitization using a green laser provides a potential novel treatment for T. rubrum infections. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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