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Keywords:

  • Scanning microscopy;
  • nonlinear microscopy;
  • coherent anti-Stokes Raman scattering;
  • biomaterials;
  • cellulose;
  • mineralogy

Abstract

A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700–4000 cm-1). Here we extend this approach to the middle (1640–3300 cm-1) and fingerprint regions (850–1800 cm-1) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm-1. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)