Hyperspectral multimodal CARS microscopy in the fingerprint region

Authors

  • Adrian F. Pegoraro,

    1. Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6 Canada
    2. National Research Council of Canada, Ottawa, Ontario, K1A 0A6 Canada
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    • These authors contributed equally to this work.

  • Aaron D. Slepkov,

    1. National Research Council of Canada, Ottawa, Ontario, K1A 0A6 Canada
    2. Department of Physics and Astronomy, Trent University, Peterborough, Ontario, K9J 7B8 Canada
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    • These authors contributed equally to this work.

  • Andrew Ridsdale,

    1. Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6 Canada
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  • Douglas J. Moffatt,

    1. Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6 Canada
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  • Albert Stolow

    Corresponding author
    1. Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6 Canada
    2. National Research Council of Canada, Ottawa, Ontario, K1A 0A6 Canada
    • Phone: 613-993-7388, Fax: 613 991-3437

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Abstract

A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700–4000 cm-1). Here we extend this approach to the middle (1640–3300 cm-1) and fingerprint regions (850–1800 cm-1) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm-1. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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