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Keywords:

  • combined RS-OCT;
  • FWHM;
  • depth-sensitive Raman spectra;
  • NA

Abstract

Complete characterization of a layered tissue requires probing both the biochemical and the morphological information from its different layers at various depths. We report the development of a combined Raman spectroscopy (RS) and optical coherence tomography (OCT) system that is capable of measuring depth-sensitive Raman signal from the tissue layers imaged by the OCT. The sample arm of a real-time time-domain OCT system was modified to allow for co-alignment of the OCT with the Raman probe beam. The depth sensitivity of Raman was obtained by incorporating confocal Raman configuration that minimized out-of-focus Raman scattered light. The system was first validated using a layered phantom prepared by depositing a thin layer of paraffin over acetaminophen. A good correlation was observed between the OCT images and the Raman signal. The system was also used to record OCT and Raman images of a resected mucosal tissue sample. While OCT image showed the presence of epithelial and stromal layers, Raman spectra measured from these layers confirmed the biochemical difference between the two. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)