Combined fluorescence-Raman spectroscopic setup for the diagnosis of melanocytic lesions

Authors

  • Riccardo Cicchi,

    Corresponding author
    1. National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy
    2. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
    • Phone: +39 055 457 2482, Fax: +39 055 457 2451

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    • Equally contributed authors.

  • Alessandro Cosci,

    1. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
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    • Equally contributed authors.

  • Susanna Rossari,

    1. Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11, 50129 Florence, Italy
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  • Dimitrios Kapsokalyvas,

    1. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
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  • Enrico Baria,

    1. Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy
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  • Vincenza Maio,

    1. Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale GB Morgagni 85, 50134, Florence, Italy
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  • Daniela Massi,

    1. Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale GB Morgagni 85, 50134, Florence, Italy
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  • Vincenzo De Giorgi,

    1. Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11, 50129 Florence, Italy
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  • Nicola Pimpinelli,

    1. Division of Clinical, Preventive and Oncology Dermatology, Department of Critical Care Medicine and Surgery, University of Florence, Piazza Indipendenza 11, 50129 Florence, Italy
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  • Francesco Saverio Pavone

    1. National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy
    2. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
    3. Department of Physics, University of Florence, Via G. Sansone 1, 50019, Sesto Fiorentino, Italy
    4. International Center of Computational Neurophotonics ICON, Via Nello Carrara 1, 50019 Florence, Italy
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Abstract

Two optical fibre-based probes for spectroscopic measurements on human tissues were designed and developed. The two probes combine fluorescence and Raman spectroscopy in a multimodal approach. The fluorescence excitation was provided by two laser diodes emitting in the UV (378 nm) and in the visible (445 nm) range, while a third source in the NIR (785 nm) was used for Raman. The device was tested on freshly excised human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin. Discrimination of lesions based on their fluorescence and Raman spectra showed good correlation with the subsequent histological examination. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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