Localized micro-scale disruption of cells using laser-generated focused ultrasound

Authors

  • Hyoung Won Baac,

    1. Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA
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    • Current address: Harvard Medical School, Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, MA 02114, USA.

  • John Frampton,

    1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
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  • Jong G. Ok,

    1. Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
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  • Shuichi Takayama,

    1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
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  • L. Jay Guo

    Corresponding author
    1. Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA
    2. Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    • Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA

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Abstract

We utilize laser-generated focused ultrasound (LGFU) to create targeted mechanical disturbance on a few cells. The LGFU is transmitted through an optoacoustic lens that converts laser pulses into focused ultrasound. The tight focusing (<100 µm) and high peak pressure of the LGFU produces cavitational disturbances at a localized spot with micro-jetting and secondary shock-waves arising from micro-bubble collapse. We demonstrate that LGFU can be used as a non-contact, non-ionizing, high-precision tool to selectively detach a single cell from its culture substrate. Furthermore, we explore the possibility of biomolecule delivery in a small population of cells targeted by LGFU at pressure amplitudes below and above the cavitation threshold. We experimentally confirm that cavitational disruption is required for delivery of propidium iodide, a membrane-impermeable nucleic acid-binding dye, into cells. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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