M13 Virus based detection of bacterial infections in living hosts

Authors

  • Neelkanth M. Bardhan,

    1. Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. The David H. Koch Institute for Integrative Cancer Research at the Massachusetts Institute of Technology, Cambridge, MA, USA
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  • Debadyuti Ghosh,

    1. Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. The David H. Koch Institute for Integrative Cancer Research at the Massachusetts Institute of Technology, Cambridge, MA, USA
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  • Angela M. Belcher

    Corresponding author
    1. Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. The David H. Koch Institute for Integrative Cancer Research at the Massachusetts Institute of Technology, Cambridge, MA, USA
    3. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    • Phone: +1 (617) 324-2800, Fax: +1 (617) 258-6558===

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Abstract

We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections in vivo. We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can target and distinguish pathogenic infections of F- pili expressing and F -negative strains of E. coli. Further, in order to tune this M13-Dye complex suitable for targeting other strains of bacteria, we have used a 1-step reaction for creating an anti-bacterial antibody -M13-Dye probe. As an example, we show anti-S. aureus -M13-Dye able to target and image infections of S. aureus in living hosts, with a 3.7× increase in fluorescence over background. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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