Imaging the noncentrosymmetric structural organization of tendon with Interferometric Second Harmonic Generation microscopy

Authors

  • Maxime Rivard,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • Konstantin Popov,

    1. University of Ottawa, Department of Physics, MacDonald Hall, 150 Louis Pasteur, Ottawa, ON, K1N 6N5, Canada
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  • Charles-André Couture,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • Mathieu Laliberté,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • Antony Bertrand-Grenier,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • François Martin,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • Henri Pépin,

    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
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  • Christian P. Pfeffer,

    1. Ludwig-Maximilians-University Munich, Department of Heart Surgery, Marchioninistraße 15, 81377 Munich, Germany
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  • Cameron Brown,

    1. University of Oxford, Botnar Research Centre, NDORMS, UK
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  • Lora Ramunno,

    1. University of Ottawa, Department of Physics, MacDonald Hall, 150 Louis Pasteur, Ottawa, ON, K1N 6N5, Canada
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  • François Légaré

    Corresponding author
    1. Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada
    • Phone: 514-228-6871, Fax: 450-929-8201===

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Abstract

We report the imaging of tendon with Interferometric Second Harmonic Generation microscopy. We observe that the noncentrosymmetric structural organization can be maintained along the fibrillar axis over more than 150 μm, while in the transverse direction it is ∼1–15 μm. Those results are explained by modeling tendon as a heterogeneous distribution of noncentrosymmetric nano-cylinders (collagen fibrils) oriented along the fibrillar axis. The preservation of the noncentrosymmetric structural organization over multiple tens of microns reveals that tendon is made of domains in which the ratio between fibrils with positive and negative polarity is unbalanced. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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