Laser-activated adhesive films for sutureless median nerve anastomosis

Authors

  • Mathew Barton,

    1. School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
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  • John W. Morley,

    1. School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
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  • Marcus A. Stoodley,

    1. The Australian School of Advanced Medicine, Macquarie University, 2 Technology Place, Macquarie University, Australia
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  • Kheng-Seong Ng,

    1. School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
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  • Sabine C. Piller,

    1. School of Science and Health, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
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  • Hong Duong,

    1. The Australian School of Advanced Medicine, Macquarie University, 2 Technology Place, Macquarie University, Australia
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  • Damia Mawad,

    1. The Bioelectronics and Neuroscience BENS Research Group, The MARCS Institute, University of Western Sydney Locked Bag 1797, Penrith NSW 2751 Australia
    2. Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, UK
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  • David A. Mahns,

    1. School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
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    • Lauto and Mahns have equally contributed to this study

  • Antonio Lauto

    Corresponding author
    1. School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia
    2. The Bioelectronics and Neuroscience BENS Research Group, The MARCS Institute, University of Western Sydney Locked Bag 1797, Penrith NSW 2751 Australia
    • Phone: +61 2 4620 3235, Fax: +61 2 4620 3025

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    • Lauto and Mahns have equally contributed to this study


Abstract

A novel chitosan adhesive film that incorporates the dye ‘Rose Bengal’ (RB) was used in conjunction with a green laser to repair transected rat median nerves in vivo. Histology and electrophysiological recording assessed the impact of the laser-adhesive technique on nerves. One week post-operatively, the sham-control group (laser-adhesive technique applied on un-transected nerves) conserved the average number and size of myelinated fibres in comparison to its contralateral side and electrophysiological recordings demonstrated no significant difference with un-operated nerves. Twelve weeks after the laser-adhesive anastomoses, nerves were in continuity with regenerated axons that crossed the anastomotic site. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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