Characterization of collagen and cholesterol deposition in atherosclerotic arterial tissue using non-linear microscopy

Authors

  • Riccardo Cicchi,

    Corresponding author
    1. National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy
    2. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
    • Phone: +39 055 2308 228, Fax: +39 055 457 2451

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  • Christian Matthäus,

    1. Institute of Photonic Technology IPHT-Jena, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Tobias Meyer,

    1. Institute of Photonic Technology IPHT-Jena, Albert Einstein Straße 9, 07745 Jena, Germany
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  • Annika Lattermann,

    1. Institute of Pathology, Department of Neuropathology, Jena University Hospital, Friedrich Schiller University, Erlanger Allee 101, 07740 Jena, Germany
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  • Benjamin Dietzek,

    1. Institute of Photonic Technology IPHT-Jena, Albert Einstein Straße 9, 07745 Jena, Germany
    2. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany
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  • Bernhard R. Brehm,

    1. Catholic Clinic – Koblenz, Internal Medicine & Cardiology, Rudolf Virchow Str. 9, 56073 Koblenz, Germany
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  • Jürgen Popp,

    1. Institute of Photonic Technology IPHT-Jena, Albert Einstein Straße 9, 07745 Jena, Germany
    2. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany
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  • Francesco Saverio Pavone

    1. National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy
    2. European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy
    3. Department of Physics, University of Florence, Via Giovanni Sansone 1, 50019, Sesto Fiorentino, Italy
    4. International Center of Computational Neurophotonics ICON, Via Nello Carrara 1, 50019, Florence, Italy
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Abstract

Atherosclerosis is characterized by the accumulation of lipids within the arterial wall and is commonly diagnosed using standard histology. Non-linear microscopy represents a possible label-free alternative to standard diagnostic methods for imaging various tissue components. Here we employ SHG and CARS microscopy for imaging thin cross-sections of atherosclerotic arterial tissue, demonstrating that both cholesterol deposition in the lumen and collagen in the normal arterial wall can be imaged and discriminated using SHG and CARS microscopy. A simultaneous detection of both forward and backward scattered SHG signals allows distinguishing collagen fibres from cholesterol. Further analysis, based on image pattern evaluation algorithms, is used to characterize collagen organization in the healthy arterial wall against collagen found within plaques. Different values of fibre mean size, distribution and anisotropy are calculated for lumen and media prospectively allowing for automated classification of atherosclerotic lesions. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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