Raman spectroscopic evidence of tissue restructuring in heat-induced tissue fusion

Authors

  • Lei Su,

    1. Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK
    2. Department of Electrical Engineering & Electronics, University of Liverpool, Liverpool L69 3GJ, UK
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    • These authors contributed equally to this work.

  • Kristy L. Cloyd,

    1. Department of Materials, Imperial College London, London SW7 2AZ, UK
    2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
    3. Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK
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    • These authors contributed equally to this work.

  • Shobhit Arya,

    1. Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK
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  • Martin A. B. Hedegaard,

    1. Department of Materials, Imperial College London, London SW7 2AZ, UK
    2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
    3. Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK
    4. Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
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  • Joseph A. M. Steele,

    1. Department of Materials, Imperial College London, London SW7 2AZ, UK
    2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
    3. Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK
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  • Daniel S. Elson,

    1. Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK
    2. Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK
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  • Molly M. Stevens,

    Corresponding author
    1. Department of Materials, Imperial College London, London SW7 2AZ, UK
    2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
    3. Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, UK
    • Molly M. Stevens, Department of Materials, Imperial College London, London SW7 2AZ, UK===

      George B. Hanna, Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK===

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  • George B. Hanna

    Corresponding author
    1. Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK
    • Molly M. Stevens, Department of Materials, Imperial College London, London SW7 2AZ, UK===

      George B. Hanna, Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, London W2 1NY, UK===

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Abstract

Heat-induced tissue fusion via radio-frequency (RF) energy has gained wide acceptance clinically and here we present the first optical-Raman-spectroscopy study on tissue fusion samples in vitro. This study provides direct insights into tissue constituent and structural changes on the molecular level, exposing spectroscopic evidence for the loss of distinct collagen fibre rich tissue layers as well as the denaturing and restructuring of collagen crosslinks post RF fusion. These findings open the door for more advanced optical feedback-control methods and characterization during heat-induced tissue fusion, which will lead to new clinical applications of this promising technology. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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