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Keywords:

  • optical tweezers;
  • fluorescence microscopy;
  • confocal microscopy;
  • 3-D imaging;
  • Jurkat cells

Abstract

We demonstrate an approach to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over the position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging. This system is particularly useful in the handling and investigation of the behavior of non-adherent immune cells. We demonstrate these capabilities by imaging and manipulating living, fluorescently stained Jurkat T cells. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)