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Keywords:

  • optical tomography;
  • velocimetry;
  • microscopy;
  • zebrafish;
  • cardiovascular system

Abstract

Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)