It's in your blood: spectral biomarker candidates for urinary bladder cancer from automated FTIR spectroscopy

Authors

  • Julian Ollesch,

    1. Ruhr-Universität Bochum, Department of Biophysics ND04, Universitätsstraße 150, 44780 Bochum, Germany
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  • Margot Heinze,

    1. Ruhr-Universität Bochum, Department of Biophysics ND04, Universitätsstraße 150, 44780 Bochum, Germany
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  • H. Michael Heise,

    1. Ruhr-Universität Bochum, Department of Biophysics ND04, Universitätsstraße 150, 44780 Bochum, Germany
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  • Thomas Behrens,

    1. Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of the Ruhr-Universität Bochum IPA, Bürkle-de-la-Camp Platz 1, 44789 Bochum, Germany
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  • Thomas Brüning,

    1. Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Institute of the Ruhr-Universität Bochum IPA, Bürkle-de-la-Camp Platz 1, 44789 Bochum, Germany
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  • Klaus Gerwert

    Corresponding author
    1. Ruhr-Universität Bochum, Department of Biophysics ND04, Universitätsstraße 150, 44780 Bochum, Germany
    • Phone: +00 49 234 32 24461, Fax: +00 49 234 32 14238

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Abstract

Blood samples of urinary bladder cancer (UBC) patients and patients with urinary tract infection were analysed with advanced automated high throughput Fourier transform infrared (HT-FTIR)-spectroscopy. Thin dried film samples were robotically prepared on multi-well titer plates (MTP) for absorbance measurements in transmission mode. Within the absorbance, 1st and 2nd derivative spectra of serum and two plasma preparations, discriminative patterns were identified and validated using bioinformatic tools. The optimal spectral resolution for data acquisition was determined. An accurate discrimination of the patient groups was achieved with three different independent spectral variable sets. The HT-FTIR blood test may support future clinical diagnostics. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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