Hyperspectral darkfield microscopy of PEGylated gold nanoparticles targeting CD44-expressing cancer cells

Authors

  • Sergiy Patskovsky,

    1. École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec, Canada
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  • Eric Bergeron,

    1. École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec, Canada
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  • Michel Meunier

    Corresponding author
    1. École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec, Canada
    • École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec, H3C 3A7, Canada

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Abstract

We present a new hyperspectral darkfield imaging system with a scanned broadband supercontinuum light source. We observed the specific attachment of the functionalized gold plasmonic nanoparticles (AuNPs) targeting CD44+ human breast cancer cells by conventional and by proposed hyperspectral darkfield microscopy. This wide-field and low phototoxic hyperspectral imaging system has been successful for performing spectral three-dimensional (3D) localization and spectroscopic identification of CD44-targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic-based imaging, disease detection and treatment in complex biological environment. Presented system capability for 3D NP tracking will also enable investigation of specific sub-cellular activity with the use of NPs as spectral sensors. (© 2013 WILEY-VCH Verlag GmbH &Co. KGaA, Weinheim)

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