Response of normal female human osteoblasts (NHOst) to 17β-estradiol is modulated by implant surface morphology

Authors

  • C. H. Lohmann,

    1. Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    2. Department of Orthopaedics, Universität Hamburg-Eppendorf, D20246 Hamburg, Germany
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  • E. M. Tandy,

    1. Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    2. Wilford Hall Medical Center, Lackland Air Force Base, Texas 78236
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  • V. L. Sylvia,

    1. Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
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  • A. K. Hell-Vocke,

    1. Department of Pediatric Orthopaedics, University Children's Hospital of Basel, CH-4005 Basel, Switzerland
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  • D. L. Cochran,

    1. Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
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  • D. D. Dean,

    1. Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
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  • B. D. Boyan,

    Corresponding author
    1. Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    2. Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    3. Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    • Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
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  • Z. Schwartz

    1. Department of Orthopaedics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    2. Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas 78229-3900
    3. Department of Periodontics, Hebrew University Hadassah Faculty of Dental Medicine, Jerusalem, Israel 91-010
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Abstract

Titanium (Ti) surfaces with rough microtopographies enhance osteogenic differentiation, local factor production, and response to osteogenic agents in vitro and increase pullout strength of dental implants in vivo. Estrogens regulate bone formation, resorption, and remodeling in females and may be important in implant success. Here, we tested the hypothesis that estrogen modulates osteoblast response to implant surface morphology. Primary female human osteoblasts were cultured to confluence on three Ti surfaces (pretreatment, PT - Ra 0.60 μm; sandblasted and acid-etched, SLA - Ra 3.97 μm; and Ti plasma-sprayed, TPS - Ra 5.21 μm) and treated for 24 h with 10−7 or 10−8M 17β-estradiol (E2). Cell number decreased with increasing surface roughness, but was not sensitive to E2. Alkaline phosphatase specific activity of isolated cells and cell layer lysates was lower on rough surfaces. E2 increased both parameters on smooth surfaces, whereas on rough surfaces, the stimulatory effect of E2 on alkaline phosphatase was evident only when measuring cell layer lysates. Osteocalcin levels were higher in the conditioned media of cells grown on rough surfaces; E2 had no effect in cultures on the plastic surfaces, but increased osteocalcin production on all Ti surfaces. TGF-β1 and PGE2 production was increased on rough surfaces, and E2 augmented this effect in a synergistic manner; on smooth surfaces, there was no change in production with E2. The response of osteoblasts to surface topography was modulated by E2. On smooth surfaces, E2 affected only alkaline phosphatase, but on rough surfaces, E2 increased levels of osteocalcin, TGF-β1, and PGE2. These results show that normal adult human female osteoblasts are sensitive to surface microtopography and that E2 can alter this response. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 204–213, 2002

Ancillary