Protein adsorption from human plasma is reduced on phospholipid polymers
Article first published online: 13 SEP 2004
Copyright © 1991 John Wiley & Sons, Inc.
Journal of Biomedical Materials Research
Volume 25, Issue 11, pages 1397–1407, November 1991
How to Cite
Ishihara, K., Ziats, N. P., Tierney, B. P., Nakabayashi, N. and Anderson, J. M. (1991), Protein adsorption from human plasma is reduced on phospholipid polymers. J. Biomed. Mater. Res., 25: 1397–1407. doi: 10.1002/jbm.820251107
- Issue published online: 13 SEP 2004
- Article first published online: 13 SEP 2004
- Manuscript Accepted: 20 MAY 1991
- Manuscript Received: 12 DEC 1990
Protein adsorption from human plasma was investigated on phospholipid polymers, poly (2-methacryloyloxyethyl phos-phorylcholine (MPC)-co-n-butyl methacrylate (BMA)) or glass by radioim-munoassay and immunogold labeling techniques. In the present studies the focus was to determine the composition and distribution of proteins at the surface of these materials after contact with human blood plasma. On all materials, protein adsorption was detected and included identification of albumin, IgG, fibrinogen, fibronectin, Hageman factor (factor XII), factor VIII/von Willebrand factor, high-molecular-weight kininogen (HMWK) and the complement protein C5. The amount of protein adsorbed decreased with an increase in MPC composition and appeared to adsorb to the surfaces in a uniform and evenly distributed manner. Therefore, we suggest that MPC moieties play an important role in suppression of protein adsorption. From these findings, it is concluded that the reduction of protein adsorption at the blood contacting surface of phospholipid polymers may result in the inhibition of thrombus formation.